The research focuses on the interplay between the neuronal activity and the functional and topological organization of neural networks. By using reduced in vitro models of specific brain regions—such as cortical, thalamic, and hippocampal networks—implemented in 2D and 3D configurations, it aims to understand how connectivity and network architecture shape emergent electrophysiological dynamics. 

The approach is based on a tight integration between Micro-Electrode Array (MEA) experiments and computational modeling, enabling the study of population activity, functional connectivity, and structure–function relationships in both physiological and pathological conditions. 

People 

Paolo Massobrio   

Publications 

I. Donati della Lunga, F. Callegari, F. Poggio, L. Cerutti, M. Pesce, G. Lo Bello, A. Simi, M. Tedesco, P. Massobrio, M. Brofiga. Scaffold-free assembly of cortical-hippocampal circuit from modular neurospheroids: a high- throughput platform to investigate network development and dynamics. Biofabrication, in press 

M. Brofiga F. Callegari, L. Cerutti, M. Tedesco, P. Massobrio. Cortical, striatal, and thalamic populations self-organize into a functionally connected circuit with long-term memory properties. Biosensors and Bioelectronics, doi: 10.1016/j.bios.2024.116840, 2025, 

M. Brofiga, M. Pisano, M. Tedesco, A. Boccaccio, P. Massobrio. Functional inhibitory connections modulate the electrophysiological activity patterns of cortical-hippocampal ensembles, Cerebral Cortex, Vol. 32, pp. 1866-1881, doi: 10.1093/cercor/bhab318, 2022